Published on Sept. 9, 2025
Guest Feature: aMPV Spray Vaccination Checklist
As part of our commitment to supporting flock health and biosecurity, we’re pleased to share a guest resource from Best Veterinary Solutions (BVS). This practical checklist, developed by BVS veterinarians and technical specialists, outlines key steps for successful aMPV spray vaccination. This is a timely tool as turkey growers all over North America prepare for the high-risk fall and winter seasons.
1. Materials
- Live AMPV Vaccine
- Distilled Water
- Spray equipment
- Backup power (battery or gas)
- Spray stabilizer
- Waterline stabilizer
- Dye
- Isopropyl alcohol (70%)
- PPE: eye protection, face mask, hearing protection
3. Mixing Vaccine
- Confirm spray equipment is clean
- Add distilled water + vaccine stabilizer to sprayer
- Calculate and prepare 1 dose/bird
- (round up: 11,500 birds = 12,000 doses)
- With distilled water, dissolve freeze-dried vaccine into vial and pour into sprayer
- Rinse vials again with distilled water and pour into spray tank
- Add dye if not pre-mixed with stabilizer
2. Pre-Mixing Prep
- Review “Dry Run” plan (water, walking path, spray settings)
- Sanitize drinker lines
- Add buffer to drinking water - (1 pack per 256 gal)
4. Application
- Close curtains in curtain-sided barns
- Turn off fans and heaters
- Spray vaccine using pre-planned walk pattern & spray settings
- Turn on fans and heaters
- Re-open curtains
5. Clean-Up
- Rinse outside of sprayer with water
- Rinse interior with distilled water
- Run distilled water through nozzle until empty
- Let equipment air dry completely before storing
This checklist is provided courtesy of BVS Poultry Talk. It is intended as a general guide and does not replace consultation with your local technical representative or veterinarian.
